Purification and characterization of a subtilisin inhibitor from seeds of foxtail millet, Setaria italica.

inhibiting subtilisin BPN'. This paper describes purification and characterization of a subtilisin inhibitor (FMSI) from foxtail millet grain. Protein was measured by the method of Lowry et al.2) using bovine serum albumin as a standard. Protein concentrations of enzyme solutions were measured spectrophotometrically with extinction coefficients as described previously.3) The concentration of Streptomyces subtilisin inhibitor (SSI)4) solution was also measured with the extinction coefficient of E0^ =0.829 (at 276nm).5) The purity of subtilisin BPN' (Nagase Biochemicals,